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Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5
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REFERENCES
Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2.
Klapper, L N, et al. (2000) Cancer Res., 60: 3384-8 (2000)
Species Reactivity Key Applications Host Format Antibody Type
Ca, H, M, R ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated domain of erbB2/Her2 on Tyrosine 1112
Molecular Weight:
185 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr1112 in human erbB2/Her2
Modifications:
Phosphorylation
Clone:
19G5
Isotype:
IgG1
Species Reactivity:
Canine
Human
Mouse
Rat
Control:
Includes EGF treated SKOV-3 lysate as a positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Storage Conditions:
2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
P04626
Entrez Gene Number:
NM_001005862.1
NM_004448.2
Gene Symbol:
ERBB2
HER2
HER-2
NGL
herstatin
erbB-2
C-erbB-2
NEU
TKR1
p185erbB2
HER-2/neu
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
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Key Applications:
ELISA
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
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