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Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8
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REFERENCES
The duration of phorbol-inducible ErbB2 tyrosine dephosphorylation parallels that of receptor endocytosis rather than threonine-686 phosphorylation: implications for the physiological role of protein kinase C in growth factor receptor signalling
Ouyang, X, et al (1998) Carcinogenesis, 19:2013-9 (1998)
Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
Ouyang, X, et al (1996) J Biol Chem, 271:21786-92 (1996)
Species Reactivity Key Applications Host Format Antibody Type
Ca, H, M ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated erbB2 on Threonine 686
Molecular Weight:
180 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2
Modifications:
Phosphorylation
Clone:
7F8
Isotype:
IgG1
Species Reactivity:
Canine
Human
Mouse
Control:
Includes EGF treated A431 cell lysates as positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P04626
Entrez Gene Number:
NM_001005862.1
NM_004448.2
Gene Symbol:
ERBB2
HER2
HER-2
NGL
herstatin
erbB-2
C-erbB-2
NEU
TKR1
p185erbB2
HER-2/neu
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Alternate Names:
NEU; NGL; HER2; TKR1; HER-2; c-erb B2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
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UniProt Summary:
FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it al |