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Anti-20S proteasome Antibody, subunit β5i, clone LMP7-1
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REFERENCES
A third interferon-gamma-induced subunit exchange in the 20S proteasome
Groettrup, M, et al (1996) Eur J Immunol, 26:863-9 (1996)
Species Reactivity Key Applications Host Format Antibody Type
H, R WB, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-20S proteasome Antibody, subunit β5i, clone LMP7-1
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes proteasome subunit beta type 8
Molecular Weight:
30 kDa
Immunogen:
Recombinant human proteasome subunit β5i
Clone:
LMP7-1
Isotype:
IgG1
Species Reactivity:
Human
Rat
Presentation:
100 µL of concentration 2.1mg/ml purified immunoglobulin from cell culture supernatant in 100 mM NaCl, 25 mM Tris (HCl), pH 8.0, containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Storage Conditions:
Stable for 1 year at -20°C from date of shipment for up to 12 months.
UniProt Number:
P28062
Entrez Gene Number:
NM_004159.4
NM_148919.3
Gene Symbol:
PSMB8
OTTHUMP00000062981
D6S216
LMP7
RING10
D6S216E
Y2
MGC1491
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Alternate Names:
Lmp7; proteasome subunit beta type 8
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Entrez Gene Summary:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
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UniProt Summary:
FUNCTION: SwissProt: P28062 # The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
SIZE: 276 amino acids; 30354 Da
SUBUNIT: The proteasome is composed of at least 15 non identical subunits which form a highly ordered ring-shaped structure. This subunit is part of the immunoproteasome where it displaces the equivalent housekeep |