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Anti-phospho-p53 (Ser392),
clone EP155Y
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PRODUCT FAMILY INFORMATION
p53 Antibodies, Proteins & Kits
Millipore’s p53 antibodies demonstrate specificity against tumor antigen p53. See below for antibodies, kits, proteins and more for p53 research, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-p53 antibody demonstrates specificity against p53. See below for data, references and related products for p53. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, R IH(P), ICC, IP, WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-p53 (Ser392) Antibody, clone EP155Y, rabbit monoclonal
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Specificity:
This antibody recognizes p53 when phosphorylated on Ser392.
Molecular Weight:
53 kDa
Epitope:
Phosphorylated Ser392
Immunogen:
Synthetic peptide corresponding to phosphorylated Ser392 and surrounding amino acids of human p53.
Modifications:
Phosphorylation
Clone:
EP155Y
Isotype:
IgG
Background Information:
The p53 gene is mutated in approximately half of all human cancers. It is involved in the cellular response to cytotoxic stresses, and together with p19ARF, induces expression of p21Cip1, to cause cell cycle arrest. In addition, p53 is able to induce apoptosis, both by transcriptional and non-transcriptional mechanisms. The amino-terminal 83 amino acids of p53 contain the transactivation domain, as well as the region involved in transcription-independent growth suppression. The carboxy-terminal region contains the DNA-binding domain, which is regulated by three phosphorylation events, and potentially by acetylation also.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Proven to react with Human and Rat.
Application Notes:
Immunohistochemistry (Paraffin) Analysis:
Anti-phospho-p53 (Ser392) was diluted to 1:200.
Immunofluorescent Immunocytochemistry:
Cells were stained with anti-phopsho-p53 (Ser392) at a 1:250 dilution.
Immunoprecipitation:
A 1:50 dilution of a previous lot was used in IP.
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Quality Assurance:
Routinely evaluated by Western Blot in lysate from A431 treated with Calyculin A and Okadaic acid.
Western Blot Analysis:
A 1:500 dilution of this antibody was used to detect p53 phosphorylated on Ser392 in A431 treated with Calyculin A and Okadaic acid.
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Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P04637
Entrez Gene Number:
NM_000546.4
Gene Symbol:
FLJ92943
LFS1
P53
TRP53
p53
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Alternate Names:
Antigen NY-CO-13
Phosphoprotein p53
Tumor suppressor p53
p53 antigen
p53 transformation suppressor
p53 tumor suppressor
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