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Anti-p53 (CT) Antibody, clone E47, rabbit monoclonal
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Species Reactivity Key Applications Host Format Antibody Type
H, not R, not M IP, ICC, FC, WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-p53 (CT) Antibody, clone E47, rabbit monoclonal
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Specificity:
This antibody recognizes p53 at the C-terminus.
Molecular Weight:
53 kDa
Epitope:
C-terminus
Immunogen:
KLH conjugated linear peptide corresponding to p53.
Clone:
E47
Isotype:
IgG
Background Information:
The p53 gene is mutated in approximately half of all human cancers. It is involved in the cellular response to cytotoxic stresses, and together with p19ARF, induces expression of p21Cip1, to cause cell cycle arrest. In addition, p53 is able to induce apoptosis, both by transcriptional and non-transcriptional mechanisms. The amino-terminal 83 amino acids of p53 contain the transactivation domain, as well as the region involved in transcription-independent growth suppression. The carboxy-terminal region contains the DNA-binding domain, which is regulated by three phosphorylation events, and potentially by acetylation also.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with mouse or rat.
Non-Reactive Species:
Rat
Mouse
Application Notes:
Flow Cytometry:
A 1:50 dilution of a previous lot was used in FC.
Immunoprecipitation:
A 1:50 dilution of a previous lot was used in IP.
Immunocytochemistry:
A 1:100 dilution of a previous lot was used in IC.
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Quality Assurance:
Routinely evaluated by Western Blot on A431 whole cell lysate.
Purification Method:
Unpurified
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P04637
Entrez Gene Number:
NM_000546.4
Gene Symbol:
p53
FLJ92943
LFS1
P53
TRP53
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Alternate Names:
Antigen NY-CO-13
Phosphoprotein p53
Tumor suppressor p53
p53 antigen
p53 transformation suppressor
p53 tumor suppressor
transformation-related protein 53
tumor protein p53
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Immunocytochemistry
Flow Cytometry
Western Blotting
Entrez Gene Summary:
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells
and at a high level in |