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Anti-CREB, clone E306, rabbit monoclonal
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PRODUCT FAMILY INFORMATION
CREB Antibodies & Kits
Millipore’s CREB Antibodies demonstrate specificity against CREB, which acts through cAMP response elements. See below for related products for CREB based on the expertise of Upstate & Chemicon.
Millipore’s Anti- CREB Antibodies demonstrates specificity against CREB. See below for data, references and related products for CREB. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IF, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-CREB Antibody, clone E306, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes CREB.
Molecular Weight:
~43 kDa
Epitope:
Internal
Immunogen:
KLH conjugated to CREB linear peptide.
Clone:
E306
Isotype:
IgG
Background Information:
CREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, rat and mouse.
Application Notes:
Flow Cytometry: 1:40 dilution from a previous lot was used on flow cytometry.
Immunohistochemistry: 1:250-500 dilution from a previous lot was used on immunohistochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
Immunocytochemistry: 1:250-500 dilution from a previous lot was used on immunocytochemistry.
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Control:
A431 whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:1000 dilution of this lot was used to detect CREB in A431 whole cell lysate.
Presentation:
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions: |