|
PDGF-AB, human recombinant
A growth factor that regulates cell growth and division
Product Overview
Alternate Names / Synonyms: Human PDGF-AB, h-PDGF-AB, PDGF-AB, PDGFAB, PDGF AB, rh-PDGF-AB, recombinant human PDGF-AB, recombinant PDGF-AB, PDGF
Gene Symbol:
Accession #:
Gene ID:
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives
Molecular Weight: 25.5 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10⁶ units/mg.
Reconstitution Instructions: Reconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |