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Leptin, rat recombinant
Adipose-derived hormone which regulates energy intake & energy expenditure
Product Overview
Alternate Names / Synonyms: rat Leptin, r-Leptin, rr-Leptin, recombinant rat Leptin, recombinant Leptin
Gene Symbol: LEP
Accession #: P50596
Gene ID: 25608
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 4.5 µM NaHCO3.
Molecular Weight: 16.2 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |