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Leptin, murine recombinant
Adipose-derived hormone which regulates energy intake & energy expenditure
Product Overview
Alternate Names / Synonyms: Murine Leptin, m-Leptin, rm-Leptin, recombinant murine Leptin, recombinant Leptin
Gene Symbol: LEP
Accession #: P41160
Gene ID: 16846
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives
Molecular Weight: 16.2 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: <0.1 ng/μg
Biological Activity: Determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Reconstitution Instructions: Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Murine Leptin is a 16 kDa protein containing 147 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |