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IL-17A, human recombinant
A proinflammatory cytokine produced by activated T cells
Product Overview
Alternate Names / Synonyms: Human IL-17A, IL-17A, IL17A, IL 17 A, h-IL-17A, rh-IL-17A, recombinant human IL-17A, recombinant IL-17, IL, interluekin
Gene Symbol: IL17
Accession #: Q16552
Gene ID: 3605
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 31.0 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts is < 2 ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intraCellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Recombinant IL-17 is a 31 kDa disulfide-linked homodier of two 136 amino acid polypeptide chains.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |