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说明：
pUC18 and pUC19 vectors are small, high copy number, E.coli plasmids, 2686 bp in length. They are identical except that they contain multiple cloning sites (MCS) arranged in opposite orientations.
pUC18/19 plasmids contain:
1 – the pMB1 replicon rep responsible for the replication of plasmid (source – plasmid pBR322). The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in the replicon rep of pMB1;
2 – the bla gene, coding for beta-lactamase, that confers resistance to ampicillin (source – plasmid pBR322). It differs from that of pBR322 by two point mutations;
3 – the region of E.coli lac operon containing a CAP protein binding site, promoter Plac, lac repressor binding site and the 5’-terminal part of the lacZ gene encoding the N-terminal fragment of beta-galactosidase (source – M13mp18/19). This fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic (alpha) complementation with a defective form of beta-galactosidase encoded by the host (mutation delta(lacZ)M15). In the presence of IPTG, bacteria synthesize both fragments of the enzyme and form blue colonies on media with X-Gal.
特性：
Purified by chromatography using proprietary patented technology.
More than 90% in the supercoiled form.
Isolated from E.coli (dam+, dcm+).
For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation see free on-line tool REviewer™.
应用：
Cloning.
Sequencing of insert DNA.
pUC18 DNA: Preparation of DNA molecular weight standards. |