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说明：
pTZ19R/U are small phagemids 2862 bp in length, constructed by inserting the DNA of phage f1 intergenic region (IG) into pUC19 and the T7 promoter sequence near to the MCS of pUC19. The phagemids differ in the orientation of the cloned f1 IG region. They are designed for DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis and in vitro transcription in one system. A host strain harboring these phagemids will produce single-stranded DNA if superinfected with the helper phage M13K07.
pTZ19R/U phagemids contain:
1 – the pMB1 replicon rep responsible for the replication of phagemid (source – plasmid pUC19);
2 – the bla gene, coding for beta-lactamase, that confers resistance to ampicillin (source – plasmid pUC19);
3 – the region of E.coli operon lac containing a CAP protein binding site, promoter Plac, lac repressor binding site and the 5’-terminal part of lacZ gene encoding the N-terminal fragment of beta-galactosidase (source – pUC19). This fragment allows blue/white screening of recombinant phagemids in the same way as described in the pUC18/19 section;
4 – a T7 promoter inserted near to the MCS of pUC19 allowing in vitro synthesis of large amounts of specific RNA;
5 – the phage f1 intergenic region carrying the sequences required in cis for initiation and termination of phage f1 DNA synthesis (+ and - strands) and for packaging DNA into bacteriophage particles.
特性：
Purified by chromatography using proprietary patented technology.
More than 90% in the supercoiled form.
Isolated from E.coli (dam+, dcm+).
For DNA sequence, sequence analysis and map creation see free on-line tool REviewer™.
应用：
Cloning.
Sequencing of insert DNA.
in vitro transcription. |