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Description
6X TriTrack™ DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF or orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.
6X TriTrack™ DNA Loading Dye is used for conventional DNA electrophoresis (see recommendations for use).
Features
Three-color tracking of DNA migration during electrophoresis.
No DNA masking during gel exposure to UV light.
EDTA binds divalent metal ions and inhibits metal dependent nucleases.
Composition
•10 mM Tris-HCl (pH 7.6),
•0.03% bromophenol blue,
•0.03% xylene cyanol FF,
•0.15% orange G,
•60% glycerol,
•60 mM EDTA
Applications
Preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels.
Quality Control
Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
Storage
Store at room temperature or at 4°C up to 12 months.
For longer periods, store at -20°C. |