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说明：
6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependant nucleases (see recommendations for use).
组分：
•0.03% bromophenol blue,
•0.03% xylene cyanol FF,
•60% glycerol,
•1% SDS,
•100 mM EDTA (pH 7.6, adjusted with Tris)
特性：
1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends.
100 mM EDTA inhibits metal-dependent nucleases.
应用：
Analysis of DNA samples with high amounts of DNA binding proteins.
Kinetic experiments.
DNA agarose gel analysis after DNA restriction digestions, ligation or dephosphorylation reactions.
质量控制：
Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
保存条件：
Store at room temperature or at 4°C up to 12 months.
For longer periods, store at -20°C.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |