DescriptionAminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH. Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.ApplicationsIndirect non-radioactive enzymatic labeling of RNA during in vitro transcription.General Characteristicslambdamax = 240 nm, extinction coefficient = 11.9x103 M-1 x cm-1 (pH 7.0),lambdamax = 290 nm, extinction coefficient = 7.8x103 M-1 x cm-1 (pH 7.0).Quality Control•Functionally tested in in vitro transcription with T7 RNA Polymerase.•Greater than 95% purity confirmed by HPLC.•Free of endo-, exodeoxyribonuclease and ribonuclease activities.StorageStore at -20°C.