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BCIP-T
(5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt)
#R0822
5 g
Assembling Lot:
Bulk Lot:
52512
Formula:
C8H6NO4BrCIP xC7H9N
Molecular Weight:
433.6
Description BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, #R0841) to enhance blue color development. Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan.
QUALITY CONTROL
Water content <3%.
max290 5,800 (pH 7.0). |