|
说明：
BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, #R0841) to enhance blue color development.
Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (1) (see the scheme below).
应用：
Detection of alkaline phosphatase activity in:
– Southern blotting,
– Western blotting,
– Northern blotting,
– dot/slot blots,
– plaque and colony screening.
质量控制：
Less than 3% water content.
保存温度：
Store at -20°C in the dark.
注意：
Preparation of a 50 mg/ml stock solution in dimethylformamide is recommended. |