IPTG,dioxane-free|现货说明:IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (1). It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening. Ready-to-use 100 mM IPTG aqueous Solution is non-toxic, stable, 0.22 µm membrane-filtered, formulated for direct use in blue/white colony screening.应用:Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1).Induction of gene expression under control of the lac promoter.质量控制:Functionally tested in blue/white cloning assay.Purity of ≥ 99.0% confirmed by HPLC.注意:Preparation of a 100 mM (23.83 mg/ml) stock solution in water is recommended. For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media.