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ProteoJET Cytoplasmic and Nuclear Protein Extraction Kit|现货进口
说明：
The ProteoJET™ Cytoplasmic and Nuclear Protein Extraction Kit is designed for rapid stepwise isolation of native, non-denatured cytoplasmic proteins and intact nuclei from cultured cells or tissue samples. The procedure, which is based on lysis with mild detergents, is fast and simple.
The isolated proteins are ready for direct use in many proteomic or enzymatic activity applications, including immunoprecipitation, affinity purification and reporter gene assays. Cell extracts prepared using this kit are compatible with common downstream applications, including 1D and 2D electrophoresis, Western blotting and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compatible with quantification assays such as Bradford, Lowry and the BCA.
The extracted nuclei can either be stored frozen in the provided Nuclei Storage Buffer or lysed with Nuclei Lysis Reagent. The intact nuclei further may be used for isolation of chromatin, histones and nuclear RNA. Nuclear extracts are used in DNA-protein interaction assays (EMSA), 2D fractionation or other applications.
The kit contains sufficient reagents for extraction of cytoplasmic and nuclear fractions from 50 samples of 50 µl wet cell pellet or 100 mg of tissue.
特性：
Versatile – isolates intact nuclei and cytoplasmic protein fraction from the same cell or tissue sample.
High quality fractionation – no cross-contamination between cytoplasmic and nuclear fractions.
Fast – procedure takes less than one hour.
Compatible with downstream applications:
– protein quantification,
– 1D and 2D electrophoresis,
– immunoprecipitation and Western blotting,
– EMSA, enzymatic activity and reporter gene assays.
应用：
Preparation of native, non-denatured nuclear and cytoplasmic protein extracts from mammalian cultured cells or tissue samples.
存储条件：
All components of the kit, except 1 M DTT, can be stored at 4°C for at least one year. Store 1 M DTT in aliquots at -20°C. |