ProteoJET Mammalian Cell Lysis Reagent|原装现货 特性: High yield total protein extraction from mammalian cultured cells or tissue samples. Ready-to-use formulation. Convenient – room temperature. Easy-to-use – no sonication, freeze-thaw cycles. Compatible with downstream applications: – protein quantification, – 1D and 2D electrophoresis, – immunoprecipitation and Western blotting, – EMSA, enzymatic activity and reporter gene assays. 说明: ProteoJET Mammalian Cell Lysis Reagent is a ready-to-use solution designed for extraction of native, non-denatured proteins from mammalian cultured cells or tissue samples. The procedure, which is based on lysis with mild detergents at room temperature, is fast and simple: no need for sonication, freeze thaw cycles or scraping of adherent cells. The procedure yields proteins in their native, non-denatured functional state. Therefore, isolated proteins can be used directly in many proteomic and enzymatic activity applications, including immunoprecipitation, affinity purification, reporter gene assays and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compatible with quantification assays such as Bradford, Lowry and the BCA. 应用: Extraction of total native, non-denatured protein from mammalian tissues or cultured cells (adherent and suspension). 注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |