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Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)|现货进口
说明：
Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR with the Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines. The master mix includes Maxima® Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Only template and primers need to be added.
Maxima® Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. The fluorescein included in the master mix allows for the collection of Dynamic Well Factors with the Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines, but does not affect qPCR efficiency. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG).
The use of Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
应用：
Real-time PCR and RT-qPCR using SYBR® Green dye on Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines.
特性：
Specificity – Maxima® Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Sensitivity – detects low copy number targets.
Fluorescein – supplemented with fluorescein passive reference dye for use with Bio-Rad iCycler iQ®, MyiQ™ or iQ5™.
Wide linear range – accurate quantification across 9 orders of magnitude.
Reproducibility and convenience – ready-to-use 2X master mix. |