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Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)|现货进口
说明：
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR with the Bio-Rad iCycler iQ, MyiQ or iQ5 machines. The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR Green I dye and is supplemented with fluorescein passive reference dye. Only template and primers need to be added.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. The fluorescein included in the master mix allows for the collection of Dynamic Well Factors with the Bio-Rad iCycler iQ, MyiQ or iQ5 machines, but does not affect qPCR efficiency. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG).
The use of Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
应用：
Real-time PCR and RT-qPCR using SYBR Green dye on Bio-Rad iCycler iQ, MyiQ or iQ5 machines.
特性：
Specificity – Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Sensitivity – detects low copy number targets.
Fluorescein – supplemented with fluorescein passive reference dye for use with Bio-Rad iCycler iQ, MyiQ or iQ5.
Wide linear range – accurate quantification across 9 orders of magnitude.
Reproducibility and convenience – ready-to-use 2X master mix. |