Maxima Probe/ROX qPCR Master Mix (2X)|现货进口 说明: Maxima® Probe/ROX qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step RT-PCR. The master mix includes Maxima® Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima® Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye. The use of Maxima® Probe/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche. 应用: Real-time PCR using sequence-specific probes. Real-time RT-PCR using sequence-specific probes. 特性: Sensitivity – detects low copy number targets. Specificity – Maxima® Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers. Wide linear range – accurate quantification across 9 orders of magnitude. Universal – can be used with sequence-specific probes on most real-time thermal cyclers. Reproducibility and convenience – ready-to-use 2X master mix minimizes pipetting error and reduces set-up time. |