Maxima SYBR Green qPCR Master Mix (2X)|现货进口 说明: Maxima SYBR Green/ROX qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step RT-PCR. The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR Green dye and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima® Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The SYBR Green intercalating dye allows DNA detection and analysis without using sequence-specific probes. ROX serves as a passive reference dye. The use of Maxima SYBR Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche. 特性: Specificity – Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers. Sensitivity – detects low copy number targets. ROX – supplemented with ROX passive reference dye. Wide linear range – accurate quantification across 9 orders of magnitude. Universal – can be used on most real-time thermal cyclers. Reproducibility and convenience – ready-to-use 2X master mix. 应用: Real-time PCR using SYBR Green dye. Real-time RT-PCR using SYBR Green dye. |