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The VECTREX® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX® matrix is dense and sediments readily even without centrifugation.
VECTREX® Avidin DLA is useful for many applications including probe purification, cDNA and subtraction library construction, hybridization analysis, and in vitro mutagenesis. One common application is subtractive hybridization. A probe biotinylated with PHOTOPROBE® Biotin, FastTag® Biotin, 3’ EndTag™ Biotin, or 5’ EndTag™ Biotin is hybridized to a nucleic acid sample in solution. After hybridization, the mixture is incubated with a suspension of VECTREX® Avidin DLA or passed through a column containing VECTREX® Avidin DLA. The DNA or RNA which is not associated with the biotinylated probe is left in solution or passes through the column. The biotinylated probe and the nucleic acid complementary to the probe are thus separated from noncomplementary sequences. The biotinylated probe can be subsequently eluted. |