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VECTREX® Avidin DLA (MB-2021) is used for reversible binding of biotinylated nucleic acids. VECTREX® Avidin DLA is made by coupling our VECTREX® matrix to a modified form of Avidin D that has a significantly reduced binding affinity for biotin. The affinity is such that, unlike VECTREX® Avidin D, biotinylated protein, nucleic acids, or other molecules can be eluted with biotin. The matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 25 ng of biotin-labeled λ DNA per μl of 1:1 suspension.
The VECTREX® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX® matrix is dense and sediments readily even without centrifugation. |