CaspSELECTTM Caspase-7 Immunoassay Kit (Fluorometric) Product Overview Kit Summary: • Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-7 activity in microtiter plates. Features & Benefits: • Simple one-step procedure; takes only 2-4 hours • Fast and convenient Kit components: • Cell Lysis Buffer • Coating Buffer • Anti-Caspase-7 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-7) • Microtiter Plate • Adhesive Plate Cover Description: Caspases play a key role in apoptosis. The CaspSELECT™ Caspase-7 Immunoassay Kit provides an effective immunosorbent enzyme assay for the specific, quantitative detection of caspase-7 activity in microtiter plates. The assay utilizes caspase-7 polyclonal antibody to capture activated caspase-7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated caspase-7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-7. Other known caspases and non-specific proteases are not detected. Storage Conditions: -20°C Shipping Conditions: gel pack USAGE: For Research Use Only! Not For Use in Humans.