CaspSELECTTM Caspase-3 Immunoassay Kit (Fluorometric) Product Overview Kit Summary: • Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. Features & Benefits: • Simple one-step procedure; takes only ~3 hours • Fast and convenient Kit components: • Cell Lysis Buffer • Coating Buffer • Anti-Caspase-3 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-3) • Microtiter Plate • Adhesive Plate Cover Description: Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected. Storage Conditions: -20°C Shipping Conditions: gel pack USAGE: For Research Use Only! Not For Use in Humans.