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c-Jun (1-79)-GST Fusion Protein
Plays a role in cell proliferation and apoptosis
Product Overview
Alternate Names / Synonyms: c-Jun
Gene Symbol: JUN; AP1; c-Jun
Accession #: P05412
Gene ID: 3725
Source: E. coli
Appearance: Liquid
Physical Form Description: 2 mg/ml in storage buffer containing 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 2 mM EDTA, 1 mM DTT, and 50% glycerol.
Molecular Weight: 37.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: C-Jun fusion protein at a concentration of 2 µg/20 µl reaction was phosphorylated in an in vitro kinase assay with 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation was detected by Western blot with phospho-specific c-Jun antibodies.
Reconstitution Instructions: N/A
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activates c-Jun.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |