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Protein G-Biotin
Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Product Overview
Alternate Names / Synonyms: Protein G
Gene Symbol: N/A
Accession #: N/A
Gene ID: N/A
Source: E. coli
Appearance: Lyophilized
Physical Form Description: Lyophilized with no additives
Molecular Weight: 26.1 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: N/A
Reconstitution Instructions: Reconstitution in water to a concentration of 5 mg/ml gives a clear solution.
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |