产品介绍:
Hoechst 33342，也称bisBenzimide H 33342或HOE 33342。分子式为C27H28N6O•3HCl•3H2O，分子量为615.99，CAS Number 23491-52-3。
Hoechst 33342是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。
Hoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。
Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。
溶解度： Hoechst 33342溶于水，溶解度可达20mg/ml。
用途：用于细胞核染色时，推荐的Hoechst 33342工作浓度为0.5-10μg/ml。
Sigma相关资料：
Sigma-Aldrich B2261 bisBenzimide H 33342 trihydrochloride
≥98% (HPLC and TLC)
Synonym: bisBenzimide, HOE 33342, Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride
CAS Number: 23491-52-3
Linear Formula: C27H28N6O • 3HCl • xH2O
Molecular Weight: 561.93 (anhydrous basis)
Beilstein Registry Number: 1234011
EC Number: 245-691-1
MDL number: MFCD00012678
PubChem Substance ID: 24891639
Description
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Application Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Properties
assay ≥98% (HPLC and TLC)
solubility H2O: 20 mg/mL
phosphate buffers: precipitates
suitability passes application test for fluoresc