|
产品介绍:
Hoechst 33342，也称bisBenzimide H 33342或HOE 33342。分子式为C27H28N6O•3HCl•3H2O，分子量为615.99，CAS Number 23491-52-3。
Hoechst 33342是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。
Hoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。
Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。
溶解度： Hoechst 33342溶于水，溶解度可达20mg/ml。
用途：用于细胞核染色时，推荐的Hoechst 33342工作浓度为0.5-10μg/ml。
Sigma相关资料：
Sigma-Aldrich B2261 bisBenzimide H 33342 trihydrochloride
≥98% (HPLC and TLC)
Synonym: bisBenzimide, HOE 33342, Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride
CAS Number: 23491-52-3
Linear Formula: C27H28N6O • 3HCl • xH2O
Molecular Weight: 561.93 (anhydrous basis)
Beilstein Registry Number: 1234011
EC Number: 245-691-1
MDL number: MFCD00012678
PubChem Substance ID: 24891639
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Application Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Properties
assay ≥98% (HPLC and TLC)
solubility H2O: 20 mg/mL
phosphate buffers: precipitates
suitability passes application test for fluorescence
storage temp. −20°C |