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FlowCellect™ Human CD4 Naïve/Memory Kit
Description:
FlowCellect™ Human CD4 Naïve/Memory Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests / Kit
Product Overview:
The FlowCellect™ CD4 Naïve/Memory kit provides a rapid and simple method to for the identification and enumeration of CD4 T Cells, the detection and quantitation of Naïve and Memory CD4 T cell subsets in either whole blood or PBMC samples using simplified no-wash assay. The kit can be utilized on any flow cytometry system equipped with blue and red lasers. The performance of these assays on the guava easyCyte 8HT platform with the Incyte software allows for cell count information on CD3, CD4 T cells, count and percentage of cell populations expressing CD62L and CD45RA.
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Background Information:
The FlowCellect™ CD8 Naïve/Memory kit allows for the detection and quantitation of Naïve and Memory CD8 T cell populations in whole blood or PBMC samples. CD8 T cells may be divided into multiple subpopulations which include naïve and memory cell populations which are further subdivided into central memory and two effector memory subsets. The balance of the naïve, the effector and memory T cells is important to ensure an efficient immune response to new antigens, while sustaining levels of memory cells that have previously encountered pathogens. Naïve CD8 T cells are defined as CD8 T cells that are immunologically naïve and have not yet encountered their antigen and lack most effector functions. Naïve T cells proliferate rapidly upon exposure to an antigen and differentiate into effector T cells which die after a brief lifespan in vivo; only a fraction of these activated cells survive and persist long term as memory CD8 T cells. On antigenic restimulation, memory CD8 T cells respond with strong proliferation and rapid transition into effector cells, migrate to diverse peripheral sites, resulting in efficacious clearance of pathogens before the onset of disease.
CD8 T cell populations can be defined and distinguished based on phenotypic and immune-functional analyses including differing homing properties. Naive and central-memory CD8 T cells express the lymph node homing receptors CCR7 and CD62L and screen lymphoid tissue for cognate antigen.
Three CD8 memory T cell populations have been described; a central memory population, CD45RA(-) CCR7(+) CD62L(+) CD28(+ ) IL-2(+) IFNγ(-), and two effector memory populations, namely the CD45RA(-) CD62L(-) CCR7(- ) and the terminally differentiated CD45RA(+) CD62L(-) CCR7(- ). Effector-memory CD8 T cells lack expression of CCR7 and CD62L and home primarily into nonlymphoid tissues[6]. Furthermore, they contain perforin, secrete IFN-γ and tumor necrosis factor-α, are cytotoxic, and are capable of rapid effector function after stimulation.
The expression of phenotypic markers described above can be utilized to distinguish naïve, central memory and effector memory subsets. The ratios of naïve and memory CD8 T cell subsets undergo alterations with development, age, and disease. The naïve and memory CD8 T cell ratios are also altered, upon antigenic stimulation with vaccines, allergens or toxins[8-10]. The identification and quantitation of naïve and memory subpopulations is becoming increasingly important in characterizing immune response or understanding mechanism of disease.
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Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo t |