ERO1 Inhibitor II, EN460 A cell-permeable thiol reactive enone (EN) compound that selectively interacts with the active-site cysteine of reduced, active form of ERO1α and inhibits its activity (IC50 = 1.9 µM). Also prevents ERO1 re-oxidation both in vitro and in mouse embryonic fibroblasts. Activates the unfolded protein response and protects ER-stressed 293T cells. Can inhibit ERO1α even in the presence of an excess amount of competing thiols. Can inhibit ERO1α even in the presence of an excess amount of competing thiols. EN460 binding to ERO1α is shown to promote the loss of flavin adenine dinucleotide (FAD) from the holoenzyme. Its inhibitory action appears to be irreversible, however addition of FAD and tris(hydroxypropyl)phosphine (Cat. No. 598250) can restore some enzyme activity. 产品信息 Form Red solid Molar mass 460.8 Protect from Light 是的 Packaged under inert gas 是的 Purity ≥99% by HPLC Solubility DMSO Chemical formula C22H12ClF3N2O4 商店和运输信息 Storage +2°C to +8°C Ship Ambient Temperature Only Standard Handling