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TACI, human recombinant
Stimulate the activation of some transcription factors
Product Overview
Product Name: TACI, human recombinant
Alternate Name/Synonyms: Transmembrane activator and CAML interactor, TNFRSF13B
Gene Symbol: TACI
Accession #: O14836
Gene ID: 23495
Source: E. Coli
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM NaCitrate, pH 4.0 and 150 mM NaCl.
Molecular Weight: 17.8 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its ability to block human BAFF induced T2B cell survival using a concentration range of 1.0-3.0 μg/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: TACI, a member of the TNF Receptor superfamily, is expressed in the small intestine, spleen, thymus, peripheral blood leukocytes, activated T cells, and resting B cells. TACI binds to both APRIL and BAFF and can stimulate the activation of transcription factors; NF-kappaB, AP-1, and mediates calcineurin dependent activation of NF-AT (nuclear-factor of activated T cells). TACI also plays a key role in the stimulation of B and T cell function. Soluble TACI inhibits APRIL-stimulated proliferation of primary B-cells by blocking the binding of APRIL to the membrane anchored TACI receptor. Recombinant human TACI is a soluble 159 amino acid polypeptide (17.8 kDa) comprising the TNFR homologous cysteine rich extracellular domain of the TACI protein.
Amino Acid Sequence: SGLGRSRRGG RSRVDQEERF PQGLWTGVAM RSCPEEQYWD PLLGTCMSCK TICNHQSQRT CAAFCRSLSC RKEQGKFYDH LLRDCISCAS ICGQHPKQCA YFCENKLRSP VNLPPELRRQ RSGEVENNSD NSGRYQGLEH RGSEASPALP GLKLSADQV
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |