SCGF-β, human recombinant

SCGF-β, human recombinant
Supports early stages of hematopoiesis
Product Overview
Product Name: SCGF-β, human recombinant
Alternate Name/Synonyms: Stem Cell Growth Factor-beta
Gene Symbol: SCF
Accession #: P10721
Gene ID: 3815
Source: E. Coli
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.25x PBS and 1mM DTT.
Molecular Weight: 25.0 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by the dose dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is ≤7.0 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-β is a 25.0 kDa polypeptide containing 227 amino acid residues.
Amino Acid Sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.