SCGF-α, human recombinant

SCGF-α, human recombinant
Supports early stages of hematopoiesis
Product Overview
Product Name: SCGF-α, human recombinant
Alternate Name/Synonyms: Stem Cell Growth Factor-alpha
Gene Symbol: SCF
Accession #: P21583
Gene ID: 4254
Source: E. Coli
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives
Molecular Weight: 33.9 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-α is a 33.9 kDa polypeptide containing 305 amino acid residues.
Amino Acid Sequence: GHGARGAERE WEGGWGGAQE EEREREALML KHLQEALGLP AGRGDENPAG TVEGKEDWEM EEDQGEEEEE EATPTPSSGP SPSPTPEDIV TYILGRLAGL DAGLHQLHVR LHALDTRVVE LTQGLRQLRN AAGDTRDAVQ ALQEAQGRAE REHGRLEGCL KGLRLGHKCF LLSRDFEAQA AAQARCTARG GSLAQPADRQ QMEALTRYLR AALAPYNWPV WLGVHDRRAE GLYLFENGQR VSFFAWHRSP RPELGAQPSA SPHPLSPDQP NGGTLENCVA QASDDGSWWD HDCQRRLYYV CEFPF
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.