|
MCP-5/CCL12, murine recombinant
Chemoattracts / activates monocytes, activated T cells, basophils, NK cells, and immature dendritic cells
Product Overview
Product Name: MCP-5/CCL12, murine recombinant
Alternate Name/Synonyms: Monocyte Chemotactic Protein-5, CCL12
Gene Symbol: CCL12
Accession #: Q62401
Gene ID: 20293
Source: E. Coli
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Molecular Weight: 9.3 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross- reacts across species. Recombinant murine MCP-5 is a 9.3 kDa protein containing 82 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Amino Acid Sequence: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |