Midkine, murine recombinant

Midkine, murine recombinant
Plays an important regulatory role in epithelial-mesenchymal interactions
Product Overview
Product Name: Midkine, murine recombinant
Alternate Name/Synonyms: MK, NEGF-2
Gene Symbol: MDK
Accession #: P12025
Gene ID: 17242
Source: E. coli.
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.4 x PBS, pH 7.4.
Molecular Weight: 13.3 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorigenesis. Recombinant murine Midkine is a 13.3 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds.
Amino Acid Sequence: VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.