|
FGF-17, human recombinant
Promotes cellular proliferation and differentiation
Product Overview
Product Name: FGF-17, human recombinant
Alternate Name/Synonyms: Fibroblast Growth Factor-17, FGFH
Gene Symbol: FGF17
Accession #: O60258
Gene ID: FGF17
Source: E. coli.
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 8.0 and 50 mM NaCl.
Molecular Weight: 22.7 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: FGF-17 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-17 signals through the FGFR 1c, 2c, 3c, and 4. FGF-17 signals induction and patterning of embryonic brain. Recombinant human FGF-17 is a 22.7 kDa protein consisting of 195 amino acid residues.
Amino Acid Sequence: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |