sFas Receptor, human recombinant

sFas Receptor, human recombinant
Leads to down-regulation of the immune response
Product Overview
Product Name: sFas Receptor, human recombinant
Alternate Name/Synonyms: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
Gene Symbol: FASL
Accession #: P25445
Gene ID: FASL
Source: E. coli.
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Molecular Weight: 17.6 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 μg/ml in the presence of 2ng/ml of hFasL.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.
Amino Acid Sequence: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.