DKK-1, Human CellExp?, human recombinant

Human CellExp™ DKK-1, Human Recombinant
Plays an important regulatory role in the Wnt /β-catenin signaling pathway
Product Overview
Product Name: Human cellexp DKK-1, Human Recombinant
Alternate Name/Synonyms: Dickkopf-related protein-1, Dickkopf-1, SK
Gene Symbol: DKK-1
Accession #: O94907
Gene ID: 22943
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.
Molecular Weight: 35-40 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β catenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/β catenin signaling cascade. It has been suggested that by inhibiting Wnt/β catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 expressed in human 293 cells is a 35-40 kDa glycoprotein containing 235 amino-acid residues.
Amino Acid Sequence: TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.