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sCD100/Semaphorin-4D, Human Recombinant
Play a role in maintaining homeostasis in the immune system
Product Overview
Product Name: sCD100/Semaphorin-4D, Human Recombinant
Alternate Name/Synonyms: Semaphorin-4D, BB18, A8, GR3
Gene Symbol: CD100
Accession #: Q92854
Gene ID: 10507
Source: CHO cells
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Molecular Weight: 78.9 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC “cooperation”. The CD100 precursor contains 862 amino acids, including a 21 aa. signal sequence, a 713 aa. extracellular domain, a 21 aa transmembrane sequence, and a 107 aa cytoplasmic region. The extracellular sequence contains several structural features, including a 479 aa “sema” domain, a 79 aa. Ig-like sequence, and a 52 aa “Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.
Amino Acid Sequence: FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |