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SPDP Crosslinker
A cleavable heterobifunctional crosslinker.
Product Overview
Product Name: SPDP Crosslinker
Alternate Name/Synonyms: N-succinimidyl-3-(2-pyridyldithio)propionate; 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester; 3-(2-Pyridyldithio)propionic acid NHS ester
Description: KEY FEATURES:
• SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups)
• SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed
• Contains a disulfide linkage in its spacer arm with a length of 6.8 Å
• The spacer is cleavable with reducing agent, such as DTT, TCEP, etc.
APPLICATIONS:
• Reversible crosslinking of proteins
• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains
• Useful for adding sulfhydryl groups to proteins by attachment to lysine residues
• Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
Peptide Sequence: N/A
Appearance: White to Off-white Solid
Formulation: N/A
CAS Number: 68181-17-9
Molecular Formula: C₁₂H₁₂N₂O₁₀
Molecular Weight: 312.36
Purity: ≥90% by NMR
Solubility:DMSO or DMF
Storage Temp.: -20 ̊C
Shipping Conditions: Gel Pack
Handling: Protect from moisture
SMILES: C1CC(=O)N(C1=O)OC(=O)CCSSC2=CC=CC=N2
InChi: InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
InChi Key: JWDFQMWEFLOOED-UHFFFAOYSA-N
PubChem CID: 100682
MDL Number: MFCD00009636
USAGE: For Research Use Only! Not For Use in Humans. |