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CD3 Monoclonal Antibody (Clone OKT3)
Monoclonal antibody to detect CD3 in human samples.
Product Overview
Product Name: CD3 Monoclonal Antibody (Clone OKT3)
Antibody Target: CD3
Alternative Name: CD3
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG2a, kappa
Immunogen: Human PBMCs
Accession#: P09693
Gene ID: 917
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Purification: Protein G Chromatography purified antibody
Species Reactivity: Human
Application: Flow (Cell Surface)
Positive Control: FACS: Human PMBCs
Application & Usage: Flow (Cell Surface): 0.1-0.25 µg/10^6 cells.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: CD3 (T3), a complex T cell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the T cell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. Structurally, CD3 consists of four distinct membrane glycoprotein isoforms (CD3g, CD3e, CD3s and the zeta chain) to generate activation signals. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for T cell development in the thymus, proliferation, and induction of T cell-mediated immune responses against infectious agents and also in the differentiation of T cells into effector and memory populations. CD3 usually expresses in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukemia, CD3 can also be useful for the identification of T cells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. CD3 indirectly plays an important role in immunomodulation whereas the anti-CD3 antibody may be used in in vitro Treg assays to generate effector T cells.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |