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Superoxide Dismutase 3 Antibody (1H12)
Monoclonal Antibody to detect superoxide dismutase 3 in human samples.
Product Overview
Product Name: Superoxide Dismutase 3 Antibody (1H12)
Antibody Target: Superoxide Dismutase 3
Alternative Name: Superoxide dismutase, SOD3.
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG 2b
Immunogen: Recombinant human protein purified from E.coli
Accession#: P08294
Gene ID: 6649
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation purified antibody
Species Reactivity: Human
Application: Western blot,IP, IHC-P, ELISA.
Positive Control: WB analysis: BOSC23 lysates, IHC analysis : normal human rectal tissue, normal human Kidney tissue
Application & Usage: Western blot: 1:2000, IP: 1 µl, IHC-P, ELISA.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. SOD3 protects the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |