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Peroxiredoxin V Antibody (3F11)
Monoclonal Antibody to detect peroxiredoxin V in human samples.
Product Overview
Product Name: Peroxiredoxin V Antibody (3F11)
Antibody Target: Peroxiredoxin V
Alternative Name: Peroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10.
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG 1
Immunogen: Recombinant human Prx V fragment protein (without mitochondrial leader sequence) purified from E.coli.
Accession#: P30044
Gene ID: 25824
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation purified antibody
Species Reactivity: Human
Application: Western blot,IP,ICC,ELISA.
Positive Control: IP analysis : HeLa cell lysate. IHC staining : HeLa cells
Application & Usage: Western blot: 1:1000, IP: 1-2 µl, ICC: 20 µl/ml, ELISA.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx V is involved in reduction of hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |