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Peroxiredoxin III Antibody (12B)
Monoclonal Antibody to detect peroxiredoxin III in human, mouse and rat samples.
Product Overview
Product Name: Peroxiredoxin III Antibody (12B)
Antibody Target: Peroxiredoxin III
Alternative Name: Thioredoxin-dependent peroxide Reductase, mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1)
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG 1
Immunogen: Recombinant human protein purified from E.coli
Accession#: P30048
Gene ID: 10935
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation purified antibody
Species Reactivity: Human, Mouse.
Application: Western blot,ELISA, IHC-P, ICC
Positive Control: WB : HeLa, 293T, Mouse Brain, Mouse Liver lysates. ICC staining of HeLa cells, Human normal duodenal tissue, Human normal placental tissue.
Application & Usage: Western blot: 1:1000, ELISA, IHC-P, ICC: 20 µl/400 µl.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx III is involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |