CBX3 Polyclonal Antibody

CBX3 Antibody
Polyclonal antibody to detect CBX3 in human, mouse and rat samples.
Product Overview
Product Name: CBX3 Antibody
Antibody Target: CBX3
Alternative Name: HECH, HP1­GAMMA, HP1Hs­gamma, HP1γ
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Fusion protein of human CBX3
Accession#: Q13185
Gene ID: 11335
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry, Immunofluorescene
Positive Control: Western Blot: Various cell lysates
Application & Usage: Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:100, IF: 1:20 – 1:50.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; However, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino terminal chromodomain, followed by a variable hinge region and a conserved carboxy­terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri­methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins  implicated in gene silencing and heterochromatin  formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA  methyltransferases, and the p150 subunit of chromatin­assembly factor­1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post­translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.