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USP1 Polyclonal Antibody
Polyclonal antibody to detect USP1 (a DUB enzyme) in human, mouse and rat samples.
Product Overview
Product Name: USP1 Polyclonal Antibody
Antibody Target: USP1
Alternative Name: USP1, UBP
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Fusion protein of human USP1
Accession#: O94782
Gene ID: 7398
Appearance: Colorless liquid
Concentration: N/A
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: HEK-293 & HeLa cell lysates
Application & Usage: Western blot: 1:200 – 1:500 dilution, IHC: 1:50 - 1:100 dilution. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitinproteosomal mechanisms. USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNAmediated translation synthesis (TLS) during DNA repair. USP1 interaction with UAF1, a WD40 repeat containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |